As of AgamP3.6 the Affymetrix microarray platform contains Anopheles probes corresponding to 11,033 annotated protein-coding genes, equating to 82% of the genes in the genome.

Approximately 24% of Anopheles transcripts in dissected samples are absent from whole-body estimates.

A linear model was fit to male and female tissue samples. On the basis of differential expression, probes are classified as either male-biased or female-biased with a 2-fold change of intensity and statistical significance at the Q<0.05 level. Overall, 56% of genes are sexually dimorphic in at least one organ. Of the 4138 sexually dimorphic genes, 70% are detected in whole-body male and female samples, with the remaining 30% only in dissected tissues. The proportion of sexually dimorphic expression in each tissue versus total sexual dimorphism is provided below, including the ratio of female- to male-biased expression (female:male).

Testis and Ovary expression sets were derived from genes detected in either the testis or ovary. Genes were considered to have acquired sex-biased somatic expression if one or more tissues show elevated expression, using calls of differential expression between males and female where there was a significant 2-fold change of expression intensity in one sex. This analysis provided gene sets for analysing expected versus observed chromosome distributions amongst the autosomes and X chromosome via chi-squared tests. A Fisher's exact test was further used to determine over- or under-representation of single chromosomes against expected counts (highlighted in bold). Please note that there is an error in how this data was originally displayed in the MozAtlas manuscript on Y-Axis of Figure 4B.